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NBAF-Edinburgh
Sanger and next-generation sequencing for UK researchers at the GenePool hosted by the NERC Biomolecular Analysis Facility - Edinburgh
The GenePool offers sequencing service on three platforms:
Sanger (dideoxy) sequencing on ABI 3730 instruments
Illumina SOLEXA short read sequencing on Illumina HiSeq 2000 and MiSeq instruments
and offers Bioinformatics Support to NERC researchers
see http://genepool.bio.ed.ac.uk for more details
ABI 3730 (Sanger) [see infosheet]
• DNA sequencing from PCR products, plasmids and other templates up to 800 bases.
• We offer services from sequence analysis only (we run your completed reactions) to full shotgun analysis of large insert clones (including construction of shotgun libraries and assembly).
• Samples may be submitted individually or in 96 well plates.
• We also perform analysis of fluorescently labeled genotyping experiments (microsatellites or AFLP).
Illumina HiSeq 2000
• The instrument can generate up to 180 million reads per lane, and there are 8 lanes per run.
• Sequences can be from 50 bases to 100 bases
• Sequences can be generated from both ends of inserts (paired-end sequencing, 50 or 100 bases from each end)
• The instrument can generate over 300 gigabases per week.
• Applications include
de novo genome sequencing
genome resequencing and targeted genome resequencing
RNA-Seq (transcriptome sequencing)
deep SAGE (digital transcriptomics)
smallRNA sequencing (microRNA sequencing)
ChIP-Seq (chromatin immunoprecipitation sequencing)
RNA-IP_Seq (RNA immunoprecipitation squencing)
and variants thereof.
Contact us for more information and a quotation genepool@ed.ac.uk
